Food analysis

What does HPTLC stand for?

High Performance Thin Layer Chromatography

How does HPLC differ from FPLC?

HPLC has higher pressure than FPLC

Fats and oils are soluble in what kind of solvents?

Hydrophobic/non-polar solvents

In food what method is used to measure heavy minerals?

ICP-AAS or ICP-OES

Name one advantage of Kjeldahl over Dumas method.

In Dumas method, the sample size is too small compared to Kjeldahl method, so the interruption due to air has higher probability, hence greater error.

What substances in low quantity can be found in food?

Indirect additives, micronutrients, trace elements, contaminants, and residues such as vitamins, minerals, pesticides, or heavy metals

In what test is Kovac's reagent used?

Indole test of IMViC (to detect indole production from tryptophan metabolism by bacteria)

The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group; what tests are involved in the IMViC?

Indole, Methyl Red, Voges Proskauer, and Citrate

What is the most accurate and sensitive method for quantitative determination of metals and metalloids?

Inductively Coupled Plasma (ICP) Atomic Absorption spectroscopy

The degree of unsaturation of a fat is measured by the ______value.

iodine value

What common lipid analysis method involves titration with sodium thiosulfate to measure the amount of iodine consumed by the fatty acid double bonds?

Iodine Value

Which instrumental method is most commonly used to determine peroxide value in fats?

Iodometric titration.

Does IR radiation have a longer or shorter wavelength than visible light?

IR radiation has a longer wavelength than visible light.

What method used for protein analysis aids the researcher in determining relative percentage of alpha helix and beta sheets in a single protein?

IR spectroscopy, circular dichroism spectroscopy, X-ray crystallography

What is isotachophoresis?

Isotachophoresis is an electrophoresis technique where ions are separated based on their electrophoretic mobility, using a discontinuous buffer system. It allows fast and focused separation, often used in analytical chemistry and microbiology.

What function does SDS have in SDS-PAGE?

It denatures the protein by breaking disulfide bonds, making it linear.

What is the purpose of a Brix measurement?

It determines the soluble solids (mainly sugars) concentration in a solution, expressed as °Brix.

In statistics, what is the Arithmetic Mean?

It is what is commonly known as the average; it is the sum of all values divided by the number of values.

What is the principle of a refractometer used in measuring sugar concentration?

It measures the refractive index of a solution, which correlates with solute concentration.

Why is titratable acidity an important measurement in food products?

It predicts the acid's impact on flavor in a food product