Card 201
Question
What is the major rate limiting or regulatory enzyme in glycolysis?
Answer
Phosphofructokinase
Biotechnology
Discover Biotechnology: 263 flashcards with questions and answers.
- Subject
- Sciences / Chemistry
- Language of creation
- English
263 flashcards
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Cards in this set
Card 202
Question
What muscle enzyme catalyzes the formation of glucose-1-phosphate from glycogen?
Answer
Phosphorylase
Card 203
Question
What is the common vector for inserting foreign genes into a bacteria?
Answer
Plasmid
Card 204
Question
What is the circular, single stranded exonuclear DNA known as?
Answer
Plasmid
Card 205
Question
What name is given to the small circular DNA molecules that carry genetic information independently of the main bacterial chromosome?
Answer
Plasmids
Card 206
Question
What alternative-protein production approach uses genetically engineered microorganisms to produce animal-identical proteins without animals?
Answer
Precision fermentation.
Card 207
Question
What fermentation approach uses microorganisms to produce specific functional proteins?
Answer
Precision fermentation.
Card 208
Question
Name 2 ways of transporting solutes across a membrane.
Answer
Pressure and electrostatic forces (concentration or electrochemical gradient are both acceptable)
Card 209
Question
What is an enzymatic digestion of animal tissues, which is commonly used in the prep of culture media for the production of toxins, and in the fermentation industry for starter cultures?
Answer
Proteose peptone
Card 210
Question
What is the substrate for lactate dehydrogenase in glycolysis to produce lactate?
Answer
Pyruvate
Card 211
Question
The final product of glycolysis is?
Answer
Pyruvate
Card 212
Question
Why is real time PCR also known as qPCR?
Answer
qPCR stands for quantitative PCR. It quantifies the amount of DNA (or cDNA) in real-time during the amplification process using fluorescent dyes or probes that emit signals as the DNA amplifies.
Card 213
Question
What is the process of cell–cell communication that allows bacteria to share information about cell density and adjust gene expression accordingly?
Answer
Quorum sensing
Card 214
Question
In genetic engineering, what is the name of the cell that receives the DNA?
Answer
Recipient cell
Card 215
Question
The first rDNA food additive sold in the USA was?
Answer
Recombinant chymosin
Card 216
Question
What is the difference between recombinant bovine growth hormone and natural bovine growth hormone?
Answer
Recombinant version has an additional Methionine
Card 217
Question
What is the function of glucagon?
Answer
Release glucose from storage to raise blood sugar levels (glycogenolysis)
Card 218
Question
What type of gene identifies which cells have been transformed in gene transfer work?
Answer
Reporter gene
Card 219
Question
What enzyme is used to cut DNA at specific recognition sequences in genetic engineering?
Answer
Restriction endonuclease.
Card 220
Question
In genetic engineering, what type of enzyme is used to cut DNA at specific nucleotide sequences, which is fundamental to creating recombinant DNA?
Answer
Restriction Endonucleases (or Restriction Enzymes)
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